;Liposomes in Drug Delivery: Status and Advances; Preparation of Drug Liposomes by Thin-Film Hydration and Homogenization; Preparation of Drug Liposomes by Reverse-Phase Evaporation; Preparation of Drug Liposomes by EDTA Gradient Methods; Preparation and Characterization of Drug Liposomes by pH-Gradient Method; Preparation and Characterization of Drug Liposomes by Ammonium Sulfate Gradient; Preparation and Characterization of Drug Liposomes by Nigericin Ionophore; Lipid-Coated Cisplatin Nanoparticles for Insoluble Drug Loading; Purification Method of Drug-Loaded Liposome; Quality Evaluation of Drug-Loaded Liposomes; Coupling Methods of Antibodies and Ligands for Liposomes; Preparation of Cell Penetrating Peptides-Mediated Targeting Drug Liposomes; Preparation and Evaluation of Folate Receptor Mediated Targeting Liposomes; Preparation and Evaluation of Rivastigmine Liposomes for Intranasal Delivery; Preparation and Evaluation of Integrin Receptor-Mediated Targeting Drug Liposomes; Preparation and Evaluation of Biomineral-Binding Antibiotic Liposomes; Preparation of Anthracyclines Liposomes for Tumor-Targeting Drug Delivery; Preparation and Characterization of pH Sensitive Drug Liposomes; Preparation and Characterization of DNA Liposomes Vaccine; HER2-Specific PEGylated Immunoliposomes Prepared by Lyophilization/Rehydration Method; Fabrication and Evaluation of Dual Peptides-Modified Liposomes Coencapsulating siRNA and Docetaxel; Dual-Modified siRNA-Loaded Liposomes for Prostate Cancer Therapy; Dequalinium-Mediated Mitochondria-Targeting Drug Liposomes for the Treatment of Drug-Resistant Lung Cancer; Preparation of Multifunctional Paclitaxel Liposomes forTreatment of Brain Glioma; Preparation of Functional Vincristine Liposomes for Treatment of Invasive Breast Cancer; Application of Labeled Liposomes in Imaging and Biodistribution Observation; Application of Drug Liposomes in the Hormone Therapy; Application of Drug Liposomes in Gene Transfection

The status and advances of liposomes in drug delivery.- Preparation of drug liposomes by thin-film hydration and homogenization.- Preparation of drug liposomes by reverse phase evaporation and freeze-thawing.- Preparation of drug liposomes by pH gradient and ammonium sulfate gradient methods.- Preparation of drug liposomes by sulfate gradient and nigericin Ionophore methods.- Preparation of drug liposomes by EDTA gradient methods.- CaP mediated insoluble drug loading for preparation of drug liposomes.

This volume describes the protocols for fabrication of liposomal drug delivery system, and consists of two parts. The first part emphasizes the basic protocols and concepts for fabrication of drug-loaded liposomes. For new investigators, laying the groundwork is the first step. This part focuses on the fabrication details in liposomes formulations, which are the so-called small tricks while usually not disclosed in mostly published research articles. However, these processing details are crucial for the preparation of liposomes. And the second part focuses on the strategies in modified and/or functionalized liposomes to adapt to pathophysiological environment, as well as their application in treating diseases. As new analytical and synthetic technologies become available, and improved understanding of pathophysiology, various functionalized drug liposomes are developing to fit the requirements of different therapeutic purposes, exhibiting a broad range of applications. Accordingly,the objectives of this part are aimed at deeply unearthing the formulation of drug-loaded liposomes, describing the detailed operation of liposomal formulation, and further demonstrating their potential applications. Therefore, this volume shows the features of experimental report or protocol for proving a guide to scientists, research students, and young investigators in pharmaceutical enterprises.

This volume mainly describes the protocols for fabrication of liposomal drug delivery system, and consists of three parts, namely, the advances in liposome-based drug delivery system, the general protocols for fabrication of drug-loading liposomes, and the protocols for preparation of functionalized liposome-based drug delivery system. It unearths the formulation of drug-loading liposomes, describes the detailed operation of liposomal formulation, and demonstrates their potential applications. This handbook is targeted at scientists, research students, and young investigators in pharmaceutical enterprises.

Prof. Dr. Wan-Liang Lu a principal investigator in State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Systems, and School of Pharmaceutical Sciences, Peking University, Beijing, China Prof. Dr. Xian-Rong Qi a principal investigator in Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Systems, Department of Pharmaceutics, and School of Pharmaceutical Sciences, Peking University, Beijing, China

Prof. Dr. Wan-Liang Lu is Professor, Deputy Chair of Department of Pharmaceutics, and Vice Dean of School of Pharmaceutical Sciences at Peking University. He received his Ph.D. degree from China Pharmaceutical University. He was a Research Scientist at National University of Singapore (NUS)/National University Hospital (NUH), Johns Hopkins Singapore International Medical Centre (2001-2003), and Toyama Medical & Pharmaceutical University (2004-2005). He is Associate Director of Pharmaceutics Specialty Committee at Chinese Pharmaceutical Association, the member of editorial board for Journal of Peking University (Health Sciences), Chinese Journal of Pharmaceutical Sciences, Asian Journal of Pharmaceutical Sciences, Chinese Pharmaceutical Journal and the referee for international peer-reviewed journals related to cancer research and pharmaceutical sciences. His research on drug delivery, cancer multi-drug resistance, pharmacokinetics, and cancer pharmacology resulted in 150 research papers. He was the recipient of Natural Science Award of China Education Ministry (1st prize) in the year 2009 and 2012.

Prof. Dr. Xian-Rong Qi is a Professor of the School of Pharmaceutical Sciences, Peking University. She received her Bachelor degree from Beijing Medical University (current Peking University School of Pharmaceutical Science, China) in 1987, and Ph.D. degree from Hoshi University (Japan, 1997). Prof. Qi has received several international and internal honors and awards, including the Nagai Foundation (1995 and 2009), Science Progress Award of Beijing Government (2001), Science Progress Award of Ministry of Education (1995,2000,2003, 2009 and 2012, respectively), Science and Technology Award of Chinese Association of Integrative Medicine (2007), and so on. Professor Qi is the principal investigator for series grants, including the Grant for the Returned Overseas Scholars of Ministry of Education, Natural Science Foundation of China, Key Grant of Ministry of Science and Technology, Natural Science Foundation of Beijing, Research Fund for the Doctoral Program of Higher Education of China, etc. She has published more than 100 research papers, and 19 textbooks.